Spectroscopy was originally the study of the interaction between radiation and matter as a function of wavelength (λ). In fact, historically, spectroscopy referred to the use of visible light dispersed according to its wavelength, e.g. by a prism. Later the concept was expanded greatly to comprise any measurement of a quantity as a function of either wavelength or frequency. Thus it also can refer to a response to an alternating field or varying frequency (ν). A further extension of the scope of the definition added energy (E) as a variable, once the very close relationship E = hν for photons was realized (h is the Planck constant). A plot of the response as a function of wavelength—or more commonly frequency—is referred to as a spectrum; see also spectral linewidth.

Spectrometry is the spectroscopic technique used to assess the concentration or amount of a given chemical (atomic, molecular, or ionic) species. In this case, the instrument that performs such measurements is a spectrometer, spectrophotometer, or spectrograph.

Spectroscopy/spectrometry is often used in physical and analytical chemistry for the identification of substances through the spectrum emitted from or absorbed by them.

Physics (Greek: physis – φύσις meaning "nature") is a natural science; it is the study of matter and its motion through spacetime and all that derives from these, such as energy and force. More broadly, it is the general analysis of nature, conducted in order to understand how the world and universe behave.

Physics is one of the oldest academic disciplines, perhaps the oldest through its inclusion of astronomy. Over the last two millennia, physics had been considered synonymous with philosophy, chemistry, and certain branches of mathematics and biology, but during the Scientific Revolution in the 16th century, it emerged to become a unique modern science in its own right. However, in some subject areas such as in mathematical physics and quantum chemistry, the boundaries of physics remain difficult to distinguish.

Physics is both significant and influential, in part because advances in its understanding have often translated into new technologies, but also because new ideas in physics often resonate with the other sciences, mathematics and philosophy.

Optics is the branch of physics which studies the behavior and properties of light, including its interactions with matter and the construction of instruments that use or detect it. Optics usually describes the behavior of visible, ultraviolet, and infrared light. Because light is an electromagnetic wave, other forms of electromagnetic radiation such as X-rays, microwaves, and radio waves exhibit similar properties.

Most optical phenomena can be accounted for using the classical electromagnetic description of light. Complete electromagnetic descriptions of light are, however, often difficult to apply in practice. Practical optics is usually done using simplified models. The most common of these, geometric optics, treats light as a collection of rays that travel in straight lines and bend when they pass through or reflect from surfaces. Physical optics is a more comprehensive model of light, which includes wave effects such as diffraction and interference that cannot be accounted for in geometric optics. Historically, the ray-based model of light was developed first, followed by the wave model of light. Progress in electromagnetic theory in the 19th century led to the discovery that light waves were in fact electromagnetic radiation.