Besançon Cathedral (Cathédrale Saint-Jean de Besançon) is a Roman Catholic cathedral located in the town of Besançon, France. It is the seat of the Archbishop of Besançon.

The cathedral is sited near the base of Mont Saint-Étienne, below the citadel. To the east of the cathedral is the 16th century Porte Rivotte, with two round towers, and pedestrian walkways dating to the 19th century. To the west is the Porte Noire, a Roman triumphal arch of the 2nd century with extensive sculptural decoration.

The cathedral consists of a large nave between two aisles, and dates from the 11th to the 13th century, with an 18th century quire.

It contains several valuable paintings, including a "Virgin and Child with Saints" by Fra Bartolommeo (1518). It also has an unfinished alabaster Pietà by the 16th century artist Conrat Meit.

In the clock tower there is a noteworthy 19th century astronomical clock with thousands of moving parts and several animated functions.