Sant'Ambrogio e Carlo al Corso (usually known simply as San Carlo al Corso) is a basilica church in Rome, Italy, facing onto the central part of the Via del Corso. It is dedicated to Saint Ambrose of Milan and Saint Charles Borromeo, also a native of that city. It is one of at least three churches in Rome dedicated to Borromeo, others include San Carlo ai Catinari and San Carlo alle Quattro Fontane.

Its construction was begun in honour of the canonization of St. Charles Borromeo in 1610, under the direction of Onorio Longhi and, after his death, of his son Martino Longhi the Younger. The site was that of the former church of San Nicola de Tofo. The ground plan is based on the Latin cross. The dome, resembling that of Santi Luca e Martina, was designed by Pietro da Cortona (1668), who was also responsible for the apse and rich internal decorations. The façade was designed by Cardinal Luigi Alessandro Omodei, who did not like the project prepared by Carlo Rainaldi.

The central vault is frescoed with a Fall of the Rebel Angels (1677–1679) by Giacinto Brandi. The altarpiece, depicting the Saints Ambrose and Charles, was painted around 1685-1690 by Carlo Maratta.